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Scott W Stevens
Associate Professor
Molecular Biosciencesscott.stevens@austin.utexas.edu
Phone: 512-232-9303
Office Location
NMS 1.262
Postal Address
2506 SPEEDWAY
AUSTIN, TX 78712-
Research Summary:
Ribonucleoproteins (RNPs) function in many cellular processes and the malfunction of some of these RNPs cause serious human disease. To better understand how disease states can be corrected, our laboratory studies the ways in which RNA and protein assemble into large RNP complexes, how those complexes function, how they are rearranged in the course of their action and how they get put back together again. Using yeast genetics, biochemistry, cryo-electron microscopy and X-ray crystallography, we seek to understand the details of the structure and function of the yeast spliceosome and other RNPs. We are also experimenting in the mammalian system by designing human cells and mice to allow us to study the complex splicing reaction in these model systems as well.
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Publications:
2009 Lardelli R.M., Thompson, J.X., Yates, J.R. III, and S.W. Stevens , Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing. , RNA 16: 516-528
2007 Chen, Y.-I., Moore R. E., Ge. H.Y. , Young M. K., Lee, T. D. and S.W. Stevens, Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors , Nucleic Acids Res. 35: 3928-3944
2006 Chen, Y.-I. Maika, S. D. and S. W. Stevens , Epitope tagging of proteins at the native chromosomal locus, J. Mol. Biol. 361: 412-419
2006 Combs, D. J., Nagel, R. J., Ares, M. Jr. and S. W. Stevens, Prp43p Is a DEAH-Box Spliceosome Disassembly Factor Essential for Ribosome Biogenesis, Mol. Cell. Biol. 26: 523-534
2002 Stevens, S. W., Ryan, D. E., Ge H. Y., Moore R. E., Young M. K., Lee, T. D. and J. Abelson , Composition and Functional Characterization of the Yeast Spliceosomal Penta-snRNP, Molecular Cell 9: 41-54
2001 Stevens, S. W., Ge H. Y., Moore R. E., Young M. K., Lee, T. D. and J. Abelson , Biochemical and genetic analyses of the U5, U6 and U4/U6•U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae, RNA 7: 1543-1553
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