In September, 2011 GSAF staff presented a three-section training on using the GSAF at UT to get NGS projects done. The full presentation can be found here. In outline, we presented:

What the GSAF does - from helping with experimental design through QC and library prep and into data analysis, see what the GSAF does day-to-day.

Application examples:

1. Finding variants using "short-read" NGS platforms (Life Technologies SOLiD and Illumina HiSeq)
2. Analyzing transcriptomes for gene expression and gene variants using short-read NGS platforms and
3. de novo sequencing using both 454 and Illumina data
   we will:
   1. describe starting material requirements
   2. discuss a schematic of the prep method
   3. plot prep time, run time, and costs, and
   4. show a typical bioinformatic analysis pipeline (command names, what data looks like, etc.)

Training and links for our new Galaxy Workflow & Sample Submission system: a "How to work with the GSAF"

1. Discussing projects & experimental design
2. Getting a quote/checking the queue via Galaxy
3. Submitting your samples
4. Getting your results
5. Analyzing your data on the GSAF server and at TACC
6. Getting help

More helpful videos can be found here:

Prezi presentation #1 on a comparison of sequencing strategies (October 2011)

Prezi presentation #2 on the Utility of DNA Sequence (October 2011)

Posted Mar 2012: Abbreviated Prezi on sequencing strategies including links to YouTube videos of NGS technologies (March 2012)